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goat polyclonal antibodies  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology goat polyclonal antibodies
Goat Polyclonal Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 4. Reduced GDNF expression in Sertoli-specific Cdc42- deficient testes (A)Westernblot analysisofCXCL12in5-week-oldCdc42-deficienttestes (n= 3). (B) Western blot analysis of GDNF and <t>FGF2</t> in 2- and 5-week-old Cdc42- deficient testes (n = 6). (C) Histological analysis of Cdc42-deficient testis microinjected with a Gdnf- expressing lentivirus. (D) Western blot analysis of GDNF in 5- to 10-day-old Cdc42-deficient testes microinjected with a Gdnf-expressing lentivirus 1 week after microinjection (n = 6). (E and F) Western blot analysis of GDNF in 4-week-old Cdc42-deficient testes microinjected with lentiviruses expressing Pak1 DN (E) or Pafah1b1 shRNA (F) 1 week after microinjection (n = 10). Asterisk indicates statistical significance (p < 0.05). Bar, 20 mm (C). Stain, hematoxylin and eosin (C).
Lot J2610 Goat Anti Mouse Fgf2 Santa Cruz, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 6. Downregulation of <t>DMRT1</t> and SOX8/9 in Cdc42-deficient testis (A) Western blot analysis of DMRT1 (n = 6), SOX8/9 (n = 5), and WT1 (n = 5) in busulfan-treated testes from Cdc42-deficient mice. (B) Immunostaining of Cdc42-deficient testes with GATA4 and DMRT1, SOX8/9, or WT1 antibodies. At least 12 tubules were counted. Arrows indicate Sertoli cells lacking candidate proteins. Arrowheads indicate GATA4+ Sertoli cells with candidate protein expression. (C) Western blot analysis of GDNF and candidate proteins following in vitro PD treatment (n = 7). (D) Immunostaining of DMRT1 and SOX8/9 in WT testes following in vitro PD treatment. At least 12 tubules were counted. Arrows indicate Sertoli cells lacking candidate proteins. Arrowheads indicate GATA4+ Sertoli cells with candidate protein expression. Bar, 20 mm (B and D). Stain, Hoechst 33342 (B and D). Asterisk indicates statistical significance (p < 0.05).
Goat Anti Mouse Dmrt1 Santa Cruz, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti fgf2 antibody sc1390  (Santa Cruz Biotechnology)
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Figure 6. Downregulation of <t>DMRT1</t> and SOX8/9 in Cdc42-deficient testis (A) Western blot analysis of DMRT1 (n = 6), SOX8/9 (n = 5), and WT1 (n = 5) in busulfan-treated testes from Cdc42-deficient mice. (B) Immunostaining of Cdc42-deficient testes with GATA4 and DMRT1, SOX8/9, or WT1 antibodies. At least 12 tubules were counted. Arrows indicate Sertoli cells lacking candidate proteins. Arrowheads indicate GATA4+ Sertoli cells with candidate protein expression. (C) Western blot analysis of GDNF and candidate proteins following in vitro PD treatment (n = 7). (D) Immunostaining of DMRT1 and SOX8/9 in WT testes following in vitro PD treatment. At least 12 tubules were counted. Arrows indicate Sertoli cells lacking candidate proteins. Arrowheads indicate GATA4+ Sertoli cells with candidate protein expression. Bar, 20 mm (B and D). Stain, Hoechst 33342 (B and D). Asterisk indicates statistical significance (p < 0.05).
Goat Anti Fgf2 Antibody Sc1390, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 6. Downregulation of <t>DMRT1</t> and SOX8/9 in Cdc42-deficient testis (A) Western blot analysis of DMRT1 (n = 6), SOX8/9 (n = 5), and WT1 (n = 5) in busulfan-treated testes from Cdc42-deficient mice. (B) Immunostaining of Cdc42-deficient testes with GATA4 and DMRT1, SOX8/9, or WT1 antibodies. At least 12 tubules were counted. Arrows indicate Sertoli cells lacking candidate proteins. Arrowheads indicate GATA4+ Sertoli cells with candidate protein expression. (C) Western blot analysis of GDNF and candidate proteins following in vitro PD treatment (n = 7). (D) Immunostaining of DMRT1 and SOX8/9 in WT testes following in vitro PD treatment. At least 12 tubules were counted. Arrows indicate Sertoli cells lacking candidate proteins. Arrowheads indicate GATA4+ Sertoli cells with candidate protein expression. Bar, 20 mm (B and D). Stain, Hoechst 33342 (B and D). Asterisk indicates statistical significance (p < 0.05).
Tx Fgf2 Goat Anti Mouse Fgf2 Santa Cruz, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti fgf 2 polyclonal antibody  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology goat anti fgf 2 polyclonal antibody
Figure 6. Downregulation of <t>DMRT1</t> and SOX8/9 in Cdc42-deficient testis (A) Western blot analysis of DMRT1 (n = 6), SOX8/9 (n = 5), and WT1 (n = 5) in busulfan-treated testes from Cdc42-deficient mice. (B) Immunostaining of Cdc42-deficient testes with GATA4 and DMRT1, SOX8/9, or WT1 antibodies. At least 12 tubules were counted. Arrows indicate Sertoli cells lacking candidate proteins. Arrowheads indicate GATA4+ Sertoli cells with candidate protein expression. (C) Western blot analysis of GDNF and candidate proteins following in vitro PD treatment (n = 7). (D) Immunostaining of DMRT1 and SOX8/9 in WT testes following in vitro PD treatment. At least 12 tubules were counted. Arrows indicate Sertoli cells lacking candidate proteins. Arrowheads indicate GATA4+ Sertoli cells with candidate protein expression. Bar, 20 mm (B and D). Stain, Hoechst 33342 (B and D). Asterisk indicates statistical significance (p < 0.05).
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polyclonal goat biotinylated anti-human fgf-10 (kgf-2) antibodies  (PeproTech)
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Figure 6. Downregulation of <t>DMRT1</t> and SOX8/9 in Cdc42-deficient testis (A) Western blot analysis of DMRT1 (n = 6), SOX8/9 (n = 5), and WT1 (n = 5) in busulfan-treated testes from Cdc42-deficient mice. (B) Immunostaining of Cdc42-deficient testes with GATA4 and DMRT1, SOX8/9, or WT1 antibodies. At least 12 tubules were counted. Arrows indicate Sertoli cells lacking candidate proteins. Arrowheads indicate GATA4+ Sertoli cells with candidate protein expression. (C) Western blot analysis of GDNF and candidate proteins following in vitro PD treatment (n = 7). (D) Immunostaining of DMRT1 and SOX8/9 in WT testes following in vitro PD treatment. At least 12 tubules were counted. Arrows indicate Sertoli cells lacking candidate proteins. Arrowheads indicate GATA4+ Sertoli cells with candidate protein expression. Bar, 20 mm (B and D). Stain, Hoechst 33342 (B and D). Asterisk indicates statistical significance (p < 0.05).
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goat polyclonal anti human fgf 2  (Cell Signaling Technology Inc)
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Figure 6. Downregulation of <t>DMRT1</t> and SOX8/9 in Cdc42-deficient testis (A) Western blot analysis of DMRT1 (n = 6), SOX8/9 (n = 5), and WT1 (n = 5) in busulfan-treated testes from Cdc42-deficient mice. (B) Immunostaining of Cdc42-deficient testes with GATA4 and DMRT1, SOX8/9, or WT1 antibodies. At least 12 tubules were counted. Arrows indicate Sertoli cells lacking candidate proteins. Arrowheads indicate GATA4+ Sertoli cells with candidate protein expression. (C) Western blot analysis of GDNF and candidate proteins following in vitro PD treatment (n = 7). (D) Immunostaining of DMRT1 and SOX8/9 in WT testes following in vitro PD treatment. At least 12 tubules were counted. Arrows indicate Sertoli cells lacking candidate proteins. Arrowheads indicate GATA4+ Sertoli cells with candidate protein expression. Bar, 20 mm (B and D). Stain, Hoechst 33342 (B and D). Asterisk indicates statistical significance (p < 0.05).
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Image Search Results


Figure 4. Reduced GDNF expression in Sertoli-specific Cdc42- deficient testes (A)Westernblot analysisofCXCL12in5-week-oldCdc42-deficienttestes (n= 3). (B) Western blot analysis of GDNF and FGF2 in 2- and 5-week-old Cdc42- deficient testes (n = 6). (C) Histological analysis of Cdc42-deficient testis microinjected with a Gdnf- expressing lentivirus. (D) Western blot analysis of GDNF in 5- to 10-day-old Cdc42-deficient testes microinjected with a Gdnf-expressing lentivirus 1 week after microinjection (n = 6). (E and F) Western blot analysis of GDNF in 4-week-old Cdc42-deficient testes microinjected with lentiviruses expressing Pak1 DN (E) or Pafah1b1 shRNA (F) 1 week after microinjection (n = 10). Asterisk indicates statistical significance (p < 0.05). Bar, 20 mm (C). Stain, hematoxylin and eosin (C).

Journal: Cell reports

Article Title: Cdc42 is required for male germline niche development in mice.

doi: 10.1016/j.celrep.2021.109550

Figure Lengend Snippet: Figure 4. Reduced GDNF expression in Sertoli-specific Cdc42- deficient testes (A)Westernblot analysisofCXCL12in5-week-oldCdc42-deficienttestes (n= 3). (B) Western blot analysis of GDNF and FGF2 in 2- and 5-week-old Cdc42- deficient testes (n = 6). (C) Histological analysis of Cdc42-deficient testis microinjected with a Gdnf- expressing lentivirus. (D) Western blot analysis of GDNF in 5- to 10-day-old Cdc42-deficient testes microinjected with a Gdnf-expressing lentivirus 1 week after microinjection (n = 6). (E and F) Western blot analysis of GDNF in 4-week-old Cdc42-deficient testes microinjected with lentiviruses expressing Pak1 DN (E) or Pafah1b1 shRNA (F) 1 week after microinjection (n = 10). Asterisk indicates statistical significance (p < 0.05). Bar, 20 mm (C). Stain, hematoxylin and eosin (C).

Article Snippet: Reagent or resource Source Identifier Antibodies Rat anti-mouse CD117 (KIT) eBioscience Cat#14-1171; RRID:AB_467434; Lot E03982-1632 Rabbit anti-mouse CDC42 Abcam Cat#64533; RRID:AB_1310067; Lot GR3200090-2 Rat anti-mouse CDH1 Gift from Dr. Masatoshi Takeichi, RIKEN CDB RRID: CVCL_A6WX Rabbit anti-mouse CLDN11 Gift from Dr. Sachiko Tsukita, Teikyo University N/A Rabbit anti-mouse non-phospho (active) CTNNB1 (Ser33/37/Thr41) Cell Signaling Technology Cat#8814 (clone: D13A1); Lot 3 Rabbit anti-mouse CXCL12 Torrey Pines Biolabs Cat#201; RRID:AB_2335896; Lot 010718 Goat anti-mouse DMRT1 Santa Cruz Cat#104885X; Lot J2610 Goat anti-mouse FGF2 Santa Cruz Cat#1390; RRID:AB_631496; Lot F1113 Goat anti-mouse GATA4 Santa Cruz Cat#1237; Lot K1611 Rabbit anti-mouse GATA4 Abcam Cat#84593; RRID:AB_10670538; Lot GR275402-1 Rabbit anti-mouse GDNF Santa Cruz Cat#328; RRID:AB_631570; Lot F2713 Rabbit anti-GFP Medical & Biological Laboratories Cat#598; RRID:AB_591819; Lot 081 Goat anti-mouse GFRA1 R&D Systems Cat#AF560; RRID:AB_2110307; Lot BQE0514081 Rabbit anti-human phospho-histone H2A.X (gH2AX) Cell Signaling Technology Cat#2577; RRID:AB_2118010; Lot 4 Rabbit anti-mouse LAMININ Sigma Cat#L9393; RRID:AB_477163; Lot 054K4780 Rat anti-Occludin Gift from Dr. Sachiko Tsukita, Teikyo University N/A Mouse anti-mouse PAFAH1B1 Santa Cruz, Dallas, TX Cat#374586 (clone: H-7); RRID:AB_11008596; Lot H0217 Mouse anti-mouse PEA15A Santa Cruz, Dallas, TX Cat#166678 (clone H-3); RRID:AB_2236964; Lot B1418 Rabbit anti-mouse phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Cell Signaling Technology Cat#9101S; RRID:AB_331646; Lot 26 Rabbit anti-mouse SOX8 Thermo Fisher Scientific Cat#PA1-28072; RRID:AB_2196251; Lot UE2770328F Goat anti-mouse SOX9 Santa Cruz Cat#17341; RRID:AB_661281; Lot F1311 Rabbit anti-mouse SOX9 Santa Cruz Cat#20095; RRID:AB_661282; Lot l1897 Rabbit anti-mouse SYCP1 Abcam Cat#15090; RRID:AB_371671; Lot GR3184119-1 Rabbit anti-mouse SYCP3 Thermo Fisher Scientific Cat#PA1-16764; RRID:AB_568727; Lot WD3244703A Mouse anti-mouse TUBB3 Sigma Cat#T5076; RRID:AB_532291; Lot 047K4852 Rabbit anti-mouse Vimentin Cell Signaling Technology Cat#5741 (clone: D21H3); RRID:AB_10695459; Lot 1 Rabbit anti-mouse WT1 Santa Cruz Cat#192; RRID:AB_632611; Lot K0811 Alexa Fluor 488-conjugated donkey anti-goat IgG Thermo Fisher Scientific Cat#A-11055; RRID:AB_2534102; Lot 2059218 (Continued on next page) e1 Cell Reports 36, 109550, August 17, 2021

Techniques: Expressing, Western Blot, Microinjection, shRNA, Staining

Figure 6. Downregulation of DMRT1 and SOX8/9 in Cdc42-deficient testis (A) Western blot analysis of DMRT1 (n = 6), SOX8/9 (n = 5), and WT1 (n = 5) in busulfan-treated testes from Cdc42-deficient mice. (B) Immunostaining of Cdc42-deficient testes with GATA4 and DMRT1, SOX8/9, or WT1 antibodies. At least 12 tubules were counted. Arrows indicate Sertoli cells lacking candidate proteins. Arrowheads indicate GATA4+ Sertoli cells with candidate protein expression. (C) Western blot analysis of GDNF and candidate proteins following in vitro PD treatment (n = 7). (D) Immunostaining of DMRT1 and SOX8/9 in WT testes following in vitro PD treatment. At least 12 tubules were counted. Arrows indicate Sertoli cells lacking candidate proteins. Arrowheads indicate GATA4+ Sertoli cells with candidate protein expression. Bar, 20 mm (B and D). Stain, Hoechst 33342 (B and D). Asterisk indicates statistical significance (p < 0.05).

Journal: Cell reports

Article Title: Cdc42 is required for male germline niche development in mice.

doi: 10.1016/j.celrep.2021.109550

Figure Lengend Snippet: Figure 6. Downregulation of DMRT1 and SOX8/9 in Cdc42-deficient testis (A) Western blot analysis of DMRT1 (n = 6), SOX8/9 (n = 5), and WT1 (n = 5) in busulfan-treated testes from Cdc42-deficient mice. (B) Immunostaining of Cdc42-deficient testes with GATA4 and DMRT1, SOX8/9, or WT1 antibodies. At least 12 tubules were counted. Arrows indicate Sertoli cells lacking candidate proteins. Arrowheads indicate GATA4+ Sertoli cells with candidate protein expression. (C) Western blot analysis of GDNF and candidate proteins following in vitro PD treatment (n = 7). (D) Immunostaining of DMRT1 and SOX8/9 in WT testes following in vitro PD treatment. At least 12 tubules were counted. Arrows indicate Sertoli cells lacking candidate proteins. Arrowheads indicate GATA4+ Sertoli cells with candidate protein expression. Bar, 20 mm (B and D). Stain, Hoechst 33342 (B and D). Asterisk indicates statistical significance (p < 0.05).

Article Snippet: Reagent or resource Source Identifier Antibodies Rat anti-mouse CD117 (KIT) eBioscience Cat#14-1171; RRID:AB_467434; Lot E03982-1632 Rabbit anti-mouse CDC42 Abcam Cat#64533; RRID:AB_1310067; Lot GR3200090-2 Rat anti-mouse CDH1 Gift from Dr. Masatoshi Takeichi, RIKEN CDB RRID: CVCL_A6WX Rabbit anti-mouse CLDN11 Gift from Dr. Sachiko Tsukita, Teikyo University N/A Rabbit anti-mouse non-phospho (active) CTNNB1 (Ser33/37/Thr41) Cell Signaling Technology Cat#8814 (clone: D13A1); Lot 3 Rabbit anti-mouse CXCL12 Torrey Pines Biolabs Cat#201; RRID:AB_2335896; Lot 010718 Goat anti-mouse DMRT1 Santa Cruz Cat#104885X; Lot J2610 Goat anti-mouse FGF2 Santa Cruz Cat#1390; RRID:AB_631496; Lot F1113 Goat anti-mouse GATA4 Santa Cruz Cat#1237; Lot K1611 Rabbit anti-mouse GATA4 Abcam Cat#84593; RRID:AB_10670538; Lot GR275402-1 Rabbit anti-mouse GDNF Santa Cruz Cat#328; RRID:AB_631570; Lot F2713 Rabbit anti-GFP Medical & Biological Laboratories Cat#598; RRID:AB_591819; Lot 081 Goat anti-mouse GFRA1 R&D Systems Cat#AF560; RRID:AB_2110307; Lot BQE0514081 Rabbit anti-human phospho-histone H2A.X (gH2AX) Cell Signaling Technology Cat#2577; RRID:AB_2118010; Lot 4 Rabbit anti-mouse LAMININ Sigma Cat#L9393; RRID:AB_477163; Lot 054K4780 Rat anti-Occludin Gift from Dr. Sachiko Tsukita, Teikyo University N/A Mouse anti-mouse PAFAH1B1 Santa Cruz, Dallas, TX Cat#374586 (clone: H-7); RRID:AB_11008596; Lot H0217 Mouse anti-mouse PEA15A Santa Cruz, Dallas, TX Cat#166678 (clone H-3); RRID:AB_2236964; Lot B1418 Rabbit anti-mouse phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Cell Signaling Technology Cat#9101S; RRID:AB_331646; Lot 26 Rabbit anti-mouse SOX8 Thermo Fisher Scientific Cat#PA1-28072; RRID:AB_2196251; Lot UE2770328F Goat anti-mouse SOX9 Santa Cruz Cat#17341; RRID:AB_661281; Lot F1311 Rabbit anti-mouse SOX9 Santa Cruz Cat#20095; RRID:AB_661282; Lot l1897 Rabbit anti-mouse SYCP1 Abcam Cat#15090; RRID:AB_371671; Lot GR3184119-1 Rabbit anti-mouse SYCP3 Thermo Fisher Scientific Cat#PA1-16764; RRID:AB_568727; Lot WD3244703A Mouse anti-mouse TUBB3 Sigma Cat#T5076; RRID:AB_532291; Lot 047K4852 Rabbit anti-mouse Vimentin Cell Signaling Technology Cat#5741 (clone: D21H3); RRID:AB_10695459; Lot 1 Rabbit anti-mouse WT1 Santa Cruz Cat#192; RRID:AB_632611; Lot K0811 Alexa Fluor 488-conjugated donkey anti-goat IgG Thermo Fisher Scientific Cat#A-11055; RRID:AB_2534102; Lot 2059218 (Continued on next page) e1 Cell Reports 36, 109550, August 17, 2021

Techniques: Western Blot, Immunostaining, Expressing, In Vitro, Staining